Active Recombinant Canine PDGFA protein(Ser87-Arg196), hFc-tagged

• Cat.No.: PDGFA-1044C
• Product Overview: Recombinant Canine PDGFA (NP_001177101.1) (Ser87-Arg196) was expressed in HEK293 with the Fc region of Human IgG1 at the N-terminus.

Specification

• Source: HEK293
• Species: Canine
• Tag: N-hFc
• Protein length: Ser87-Arg196
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured in a cell prolifeRation assay using BALB/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 5-25 ng/mL.
• Molecular Mass: The recombinant Canine PDGFA consists 370 amino acids and predicts a molecular mass of 41 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.