Recombinant Chikungunya CHIKVgp2 protein

• Cat.No.: CHIKVgp2-27C
• Product Overview: Recombinant Chikungunya Mutant (A226V) E1 produced in Insect Cells is a polypeptide chain containing amino acids 1-415, however at position 226 the Alanine of the wild-type CHIKV E1 gene was mutated to Valine. The molecular weight of the CHIKV Mutant is approximately 50kDa. The E1 protein is C-terminal part of E2-6K-E1 protein region. CHIKV Mutant is purified by proprietary chromatographic technique.

Specification

• Description: Chikungunya virus (CHIKV) is an arthropod-born virus which is a member of the Alphavirus genus belonging to the Togaviridae family. CHIKV nucleocapsid is comprised of a single-stranded plussense RNA genome of approximately 11.8 kb. The CHIKV virion envelope consists of a lipid bilayer derived from the plasma membrane from the host cell, multiple copies of 2 major virus encoded glycoproteins E1 and E2, and a small 6K peptide. Proteins E1 and E2 both have a molecular weight of roughly 50kDa and form a heterodimer anchored in the membrane. Chikungunya virus infection causes an illness with symptoms similar to those of the dengue fever with an acute febrile phase lasting only 2-5 days, followed by a prolonged arthralgic disease affecting the joint extremities. Recent Chikungunya virus outbreaks presented a prospect for genetic analysis of patients with the illness, revealing a point mutation at the amino acid 226 (Ala mutated to Val) of the E1 gene. This point mutation was confirmed to be responsible for an improved capacity of CHIKV strains to infect and replicate in the Aedes albopictus, enabling virus transmission to a naive human population.
• Source: Insect cells
• Species: Chikungunya
• Form: Sterile filtered colorless solution. CHIKV Mutant protein solution in 1xD-PBS, pH7.4, 0.1% Thimerosal, 5mM EDTA, 1µg/ml of Leupeptin, Aprotinin and Pepstatin A.
• Molecular Mass: 50kDa
• Protein length: amino acids 1-415
• AA sequence: YEHVTVIPNTVGVPYKTLVNRPGYS PMVLEMELLSVTLEPTLSLDYITCE YKTVIPSPYVKCCGTAECKDKNLPD YSCKVFTGVYPFMWGGAYCFCDAEN TQLSEAHVEKSESCKTEFASAYRAH TASASAKLRVLYQGNNITVTAYANG DHAVTVKDAKFIVGPMSSAWTPFDN KIVVYKGDVYNMDYPPFGAGRPGQF GDIQSRTPESKDVYANTQLVLQRPA VGTVHVPYSQAPSGFKYWLKERGAS LQHTAPFGCQIATNPVRAVNCAVGN MPISIDIPEAAFTRVVDAPSLTDMS CEVPACTHSSDFGGVAIIKYAASKK GKCAVHSMTNAVTIREAEIEVEGNS QLQISFSTALASAEFRVQVCSTQVH CAAECHPPKDHIVNYPASHTTLGQD ISATAMSWVQKITGG.
• Purity: Protein is >95% pure as determined by 12.5% SDS-PAGE.
• Usage: The products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
• Stability: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.
  For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
• Shipping: Ice Packs

Gene Information

• Gene Name: CHIKVgp2 contains C, E3, E2, 6K, and E1 proteins [ Chikungunya virus ]
• Official Symbol: CHIKVgp2
• Synonyms: CHIKVgp2; contains C, E3, E2, 6K, and E1 proteins; E1 envelope glycoprotein; Structural polyprotein
• Gene ID: 956308
• Protein Refseq: NP_690589.2