Recombinant cynomolgus ENPP2 (XP_005564040.1) (Asp49-Ile863) was expressed with a polyhistidine tag at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS,pH7.4, 5%~8% trehalose and mannitol.
Measured by its ability to cleave Bis (p-Nitrophenyl) Phosphate (BPNPP). The specific activity is >1,000 pmol/min/ug.
The recombinant cynomolgus ENPP2 consists 834 amino acids and predicts a molecular mass of 96 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>85 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.