Active Recombinant Cynomolgus ERBB2 protein, hFc-tagged

• Cat.No.: ERBB2-1216C
• Product Overview: Recombinant Cynomolgus ERBB2 protein()(Met1-Thr652), fused with hFc tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: hFc
• Form: Lyophilized from sterile PBS, pH 7.4.Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Immobilized Her2/ERBB2 Protein, Cynomolgus, Recombinant (ECD, hFc Tag)(Cat:90295-C02H) at 2 μg/ml (100 μl/well) can bind Anti-Erbb2 Antibody (Trastuzumab)(Cat:68046-H002), the EC50 is 15-70 ng/mL.2. Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.3-1.8 μg/mL in the presence of 0.6 µg/mL Anti-ErbB2/Her2 Monoclonal Antibody.
• Molecular Mass: The recombinant cynomolgus ERBB2 consists 871 amino acids and predicts a molecular mass of 96.4 kDa.
• Protein Length: Met1-Thr652
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.