Recombinant Cynomolgus IL1B Protein

Cat.No.: IL1B-1167C
Product Overview: Recombinant mature form of Cynomolgus (Macaca fascicularis) IL1B (Ala 117-Ser 269) was expressed and purified, with an initial Met.
Source: E. coli
Species: Cynomolgus
Predicted N Terminal: Met 1
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. The ED50 for this effect is typically 3-15 pg/mL.
Molecular Mass: The recombinant Cynomolgus IL1B consists of 154 amino acids and has a calculated molecular mass of 17.5 kDa. It migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >97 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: IL1B interleukin 1 beta [ Macaca fascicularis ]
Official Symbol: IL1B
Synonyms: IL-1 beta; Interleukin-1 beta; interleukin 1, beta
Gene ID: 102119749
mRNA Refseq:
Protein Refseq:
MIM:
UniProt ID:
Chromosome Location: chromosome: 13
Pathway: AGE-RAGE signaling pathway in diabetic complications, organism-specific biosystem; Alzheimer"s disease, conserved biosystem; Amoebiasis, conserved biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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