Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant Cynomolgus Monkey CD80 protein, His-tagged

Recombinant Cynomolgus Monkey CD80 protein, His-tagged

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Cat.No.:
CD80-274C
Product Overview:
Recombinant Cynomolgus Monkey CD80(Val35-Asn242) fused with His tag at C-terminal was expressed in HEK293.
Description:
B7-1/CD80 and B7-2/CD86, together with their receptors CD28 and CTLA-4, constitute one of the dominant co-stimulatory pathways that regulate T- and B-cell responses. Although both CTLA-4 and CD28 can bind to the same ligands, CTLA-4 binds to B7-1 and B7-2 with a 20 - 100 fold higher affinity than CD28 and is involved in the down-regulation of the immune response. Mature cynomolgus B7-1 consists of a 208 aa extracellular domain (ECD) with two immunoglobulin-like domains, a 21 aa transmembrane domain, and a 25 aa cytoplasmic domain. Within the ECD, cynomolgus B7-1 shares 97%, 51%, and 54% aa sequence identity with human, mouse, and rat B7-1, respectively. Both human and mouse B7-1 and B7-2 can bind to either human or mouse CD28 and CTLA-4. B7-1 is expressed on activated B cells, activated T cells, and macrophages. B7-2 is constitutively expressed on interdigitating dendritic cells, Langerhans cells, peripheral blood dendritic cells, memory B cells, and germinal center B cells.
Source:
HEK293
Species:
Cynomolgus Monkey
Tag :
His
Predicted N Terminal:
Val35
Form:
Lyophilized from a 0.2 μm filtered solution in PBS.
Bio-activity:
Measured by its binding ability in a functional ELISA. When Recombinant Cynomolgus Monkey B7-1/CD80 is immobilized at 0.5 µg/mL (100 µL/well), the concentration of Recombinant Human CTLA-4 Fc Chimera that produces 50% of the optimal binding response is approximately 5-30 ng/mL.
Molecular Mass:
Predicted Molecular Mass: 25 kDa
SDS-PAGE: 35-45 kDa, reducing conditions
Endotoxin:
<0.1 EU per 1 μg of the protein by the LAL method.
Purity:
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage:
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution:
Reconstitute at 1 mg/mL in PBS.


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Note: There will be extra charge for optional service!
Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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