Active Recombinant Cynomolgus SELE Protein, Fc-tagged

• Cat.No.: SELE-1202C
• Product Overview: Recombinant cynomolgus SELE (EHH61734.1) (Met1-Pro556) was expressed with the Fc region of human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Cynomolgus
• Tag: Fc
• Form: Lyophilized from sterile 50 mM Tris, pH 8.0.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells.
  When 5 x 10E4 cells/well are added to Cynomolgus/Rhesus SELP/Fc Chimera coated plates (2 μg/mL, 100 μL/well), > 40% cells will adhere after 1 hour at 37°C.
• Molecular Mass: The recombinant cynomolgus SELE consists 776 amino acids and predicts a molecular mass of 85.7 kDa.
• Protein Length: Met1-Pro556
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.