Recombinant Cynomolgus TNF Protein

Cat.No.: TNF-1168C
Product Overview: Recombinant cynomolgus TNF [(Identical to the rhesus TNF (NP_001040614.1)] (Val77-Leu233) was expressed and purified with an initial Met.
Source: E. coli
Species: Cynomolgus
Predicted N Terminal: Met
Form: Lyophilized from sterile 50 mM Tris, 50 mM NaCl, pH 8.0, 5%~8% trehalose and mannitol.
Bio-activity: Measured in a cytotoxicity assay using L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 50-300 pg/ml.
Molecular Mass: The recombinant cynomolgus TNF consists of 158 amino acids and has a calculated molecular mass of 17.4 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >90 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: TNF tumor necrosis factor [ Macaca fascicularis ]
Official Symbol: TNF
Synonyms: TNFA; TNF-a; TNFSF2; Cachectin; TNF-alpha; tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha
Gene ID: 102139631
mRNA Refseq: NM_001285277
Protein Refseq: NP_001272206
MIM:
UniProt ID: P79337
Chromosome Location: chromosome: 4
Pathway: AGE-RAGE signaling pathway in diabetic complications, organism-specific biosystem; Adipocytokine signaling pathway, organism-specific biosystem; African trypanosomiasis, organism-specific biosystem

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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