Cynomolgus 4-1BB / TNFRSF9 Protein, Leu 24 - Gln 186 (Accession # XP_005544946.1) was produced in human 293 cells (HEK293).
Predicted N Terminal:
Lyophilized from sterile PBS, pH7.4, 10% Trehalose.
Measured by its binding ability in a functional ELISA. Immobilized Cynomolgus 4-1BB, His Tag at 1μg/mL (100 ul/well),can bind Human 4-1BB Ligand, Fc Tag with a linear of 0.01-0.5 ng/mL.
Cynomolgus 4-1BB, His Tag is fused with a polyhistidine tag at the C-terminus, and has a calculated MW of 19.2 kDa. The reducing protein migrates as 30-40 kDa in SDS-PAGE.
Less than 1.0 EU per μg by the LAL method.
>92% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.