Recombinant Cynomolgus (Macaca fascicularis) p53 (E3U906) (Met 1-Asp 393) was expressed and purified.
Predicted N Terminal:
Lyophilized from sterile 100mM Tris, 3% glycerol, 4mM DTT, pH 8.01, 5%~8% trehalose and mannitol.
1. Measured by its ability to bind biotinylated human S100B in a functional ELISA. 2. Measured by its ability to bind biotinylated human RRM2B in a functional ELISA.
The recombinant Cynomolgus p53 consisting of 393 amino acids and has a calculated molecular mass of 43.7 kDa. It migrates as an approximately 48 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>88 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.