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Recombinant Daucus carota CR16 protein

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E. coli(1)
Cat.No.:
CR16-369D
Product Overview:
Recombinant Daucus carota CR16 was expressed in E. coli.
Description:
Dau c 1.2 is a lyophilized, recombinant protein with IgE-binding capacity. It was produced by heterologous expression in E. coli, purified by conventional biochemical methods.
Source:
E. coli
Species:
Daucus carota
Form:
The product was lyophilized in 5 mM NH4HCO3, containing 1% sucrose.
Molecular Mass:
~17,339 Dalton
Purity:
> 99%
Storage:
The lyophilized product can be kept at room temperature for at least 3 weeks. However, we recommend the product to be stored at -20 centigrade. Under these conditions the quality of the material will be maintained for several years. The stability at 4 centigrade should be at least 6 months. Reconstituted protein can be stored at -20 centigrade.
Concentration:
1 mg/ml
Reconstitution:
The material can be reconstituted with water or diluted buffers. If reconstituted with water or buffers (2mM -Mercaptoethanol) to 2 mg/ml, the sucrose concentration will be approx. 30 mM. If reconstituted with water or buffers (2mM -Mercaptoethanol) to 2mg/ml, the product is soluble to approx. 98%. Thorough physical suspension of the protein is essential. Alternatively the product can be dissolved in 6M Urea, 1mM ß-Mercaptoethanol. The urea solution can be dialyzed against a suitable buffer (20mM Tris/HCl pH 8.0, 1mM -Mercaptoethanol) without precipitation of protein.
UniProt ID:
O04298


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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