Recombinant Dengue virus E protein, His-tagged

• Cat.No.: E-185D
• Product Overview: Recombinant Dengue virus(type 2, strain New Guinea C/PUO-218 hybrid) Envelope Protein(Phe247-Gly675) fused with two amino acids "MV" at the N-terminal) and a polyhistidine tag at the C-terminus was expressed in Insect cells.

Specification

• Description: Envelope played an important role in many functions.
• Source: Insect cells
• Species: Dengue virus
• Tag: His
• Predicted N Terminal: Met
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol.
• Molecular Mass: The recombinant envelope protein of Dengue virus (DENV)(type 2, strain New Guinea C/PUO-218 hybrid) consists of 442 amino acids and predicts a molecular mass of 49.2 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: >90 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade upon receiving. Recommend to aliquot the protein into smaller quantities for optimal storage. Avoid repeated freeze-thaw cycles.