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Home > Products > Toxin > Recombinant E. coli Heat-Labile Enterotoxin, B subunit

Recombinant E. coli Heat-Labile Enterotoxin, B subunit

"Heat-Labile Enterotoxin, B subunit" Related Products.
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E. coli(1)
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Product Overview:
Recombinant E. coli Heat-Labile Enterotoxin, B subunit was expressed in P. pastoris.
Enterotoxigenic Escherichia coli causes diarrhea through its heat-labile enterotoxin (LT). The LT is a periplasmic protein composed of one A subunit (LTA, 27 kDa) and five non-covalently associated B subunits (LTB, 11.6 kDa each) forming a ring-like pentamer. LTB has high affinity towards the toxin receptor ganglioside GM1, a glycosphingolipid found ubiquitously on the surface of mammalian cells. Ganglioside GM1 facilitates the delivery of the A subunit to the cytosol of the target cell resulting in persistent synthesis of cAMP and subsequently diarrhea. This characteristic makes LTB a good label for microglial cells (due to the enrichment of ganglioside GM1 on their cell surface). In addition, studies made mostly with animal models demonstrated that recombinant LTB could stimulate strong serum and mucosal immune responses against LT. Other studies have indicated that LTB could be used as a potent mucosal adjuvant.
P. pastoris
E. coli
Lyophilized from a solution containing Heat-Labile Enterotoxin, B subunit in 0.05 M Tris buffer, pH 7.5, 0.2 M NaCl, 3 mM NaN3, and 1 mM sodium EDTA.
The activity is measured by ELISA using ganglioside GM1-coated plates. LTB at various concentrations is incubated on the ganglioside GM1 coated wells, followed by anti-cholera toxin antibody, and peroxidase-labeled goat anti-rabbit IgG as the secondary antibody. Binding saturation of 50% may be achieved with = 0.12 μg/mL of LTB.
Molecular Mass:
11.6 kDa
>90% (SDS-PAGE)
Store at 2-8 centigrade

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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-097-1828
FAX: 1-631-938-8127

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