Recombinant Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP (AHX24649.1) (Met1-Gln650, with the deletion of Thr309-Asn484) was expressed with the bacteriophage T4 fibritin and a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
The recombinant Zaire ebolavirus(strain H.sapiens-wt/GIN/2014/Kissidougou-C15) GP consists 485 amino acids and predicts a molecular mass of 54.8 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>(12.2+31.2+56)% as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.