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Recombinant EBOV (subtype Zaire, strain Mayinga 1976) GP Protein, His-tagged

Cat.No.: GP-741V
Product Overview: Recombinant Zaire ebolavirus (strain Mayinga 1976) GP (AAC54887.1) (Met1-Phe308) was expressed with a polyhistidine tag at the C-terminus.
Source: HEK293
Species: EBOV
Tag: His
Predicted N Terminal: Ile 33
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Molecular Mass: The recombinant Zaire ebolavirus (strain Mayinga 1976) GP consists 287 amino acids and predicts a molecular mass of 32.3 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: GP virion spike glycoprotein precursor [ Zaire ebolavirus ]
Official Symbol: GP
Synonyms: spike glycoprotein; small secreted glycoprotein; second secreted glycoprotein
Gene ID: 911829
mRNA Refseq:
Protein Refseq: NP_066246
MIM:
UniProt ID: Q05320

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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