Active Recombinant Ferret CD4 Protein, His-tagged

• Cat.No.: CD4-1023F
• Product Overview: Recombinant extracellular domain of Ferret (Mustela putorius furo) CD4 (ABS50090.1) (Met 1-Leu 401) was expressed, with a polyhistidine tag at the C-terminus.

Specification

• Source: HEK293
• Species: Ferret
• Tag: His
• Predicted N Terminal: Glu 27
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of NIH-3T3 mouse embryonic fibroblast cells.When 5 × 10^4 cells/well areadded to CD4 coated plates (1.25μg/mL and 100μL/well), approximately >50% will adhere specifically after 30
• Molecular Mass: The recombinant Ferret CD4 consists of 386 amino acids and has a calculated molecular mass of 42.7 kDa. The apparent molecular mass of the recombinant protein is approximately 52 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >97 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.

Gene Information

• Gene Name: CD4 CD4 molecule [ Mustela putorius furo ]
• Official Symbol: CD4
• Synonyms: CD4 molecule
• Gene ID: 101684487
• mRNA Refseq: NM_001310164
• Protein Refseq: NP_001297093