Recombinant full length of Influenza A virus (A/Jiangxi-Donghu/346/2013(H10N8)) hemagglutinin (translated amino acid sequence of EPI497477 ) (Met1-Ile561),termed as HA, was expressed.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH7.41, 5%~8% trehalose and mannitol.
Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 30-150μg/mL for 1% GRBC.
The recombinant hemagglutinin of Influenza A virus (A/Jiangxi-Donghu/346/2013(H10N8)) consists 545 amino acids and predicts a molecular mass of 60.6 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.