Recombinant H13N6 (A/black-headed gull/Sweden/1/1999) HA Protein, His-tagged
Recombinant Influenza A virus (A/black-headed gull/Sweden/1/1999(H13N6)) hemagglutinin (AAV91213.1) (Met1-Arg342), termed as HA1, was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.1-0.5μg/mL for 1% GRBC.
The recombinant HA1 subunit of Influenza A virus (A/black-headed gull/Sweden/1/1999(H13N6)) consists 335 amino acids and predicts a molecular mass of 37.3 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.