Recombinant H13N8 (A/black-headed gull/Netherlands/1/00) HA Protein, His-tagged

• Cat.No.: HA-416V
• Product Overview: Recombinant extracellular domain (Met 1-Lys 528) of the influenza A hemagglutinin H13N8 (A/black-headed gull/Netherlands/ 1/00 (H13N8)) (AAV91212.1), (HA1+HA2, uncleaved) was expressed, fused with a C-terminal polyhistidine tag.

Specification

• Source: HEK293
• Species: H13N8
• Tag: His
• Predicted N Terminal: Asp 19
• Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
• Molecular Mass: The secreted recombinant influenza A H13N8 HA (A/black-headed gull/Netherlands/1/00 (H13N8)) comprises 521 amino acids and has a predicted molecular mass of 58.5 kDa. As a result of glycosylation, the apparent molecular mass of the protein is approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.