Recombinant H15N8 (A/duck/AUS/341/1983) HA Protein, His-tagged
Recombinant extracellular domain (Met 1-Val 534) of the influenza A H15N8 hemagglutinin (A/duck/AUS/341/1983 (H15N8)) (ABB88132.1) (HA1+HA2, uncleaved) was expressed, fused with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to agglutinate human red blood cells.HA titer is 4-20 μg/mL for 1% HRBC.
The secreted recombinant influenza A H15N8 HA (A/duck/AUS/341/1983 (H15N8)) comprises 527 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 75 kDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>97 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.