Recombinant N-terminal segment (Met1-Arg345) of the influenza A hemagglutinin (A/Wisconsin/67/2005 (H3N2)) (ACF54576.1), termed as HA1, was expressed, fused with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
The secreted recombinant influenza A H3N2 HA1 subunit (A/Wisconsin/67/2005 (H3N2)) comprises 340 amino acids and has a predicted molecular mass of 38 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.