Recombinant N-terminal segment (Met 1-Arg 343) of the influenza hemagglutinin ((A/mallardduck/Alberta/299/1977 (H4N4)) (Q0A4G1), termed as HA1, was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5% ~ 8% trehalose and mannitol.
The secreted recombinant HA1 subunit of influenza A H1N1 HA ((A/mallard duck/Alberta/299/1977(H4N4)) comprises 338 amino acids and has a predicted molecular mass of 37.7 kDa. As a result of glycosylation, it migrates as an approximately 50 kDa band in SDS
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.