Recombinant H5N1 (A/VietNam/1203/2004) HA Protein, His & Fc-tagged
Recombinant extracellular domain of hemagglutinin (Influenza A virus (A/Viet Nam/1203/2004 (H5N1)) (AAW80717.1) (Met 1-Gln 531) with cleavage site mutated (RERRRKKR→TETR,HA1+HA2,uncleaved) was fused with the C-terminal polyhistidine-tagged Fc region of mouse IgG1 at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 100mM NaAc, 10mM NaCl, 200mM Tris, pH 7.5, 5%~8% trehalose and mannitol.
Recombinant HA/mFc is a disulfide-linked homodimeric protein. The reduced monomer consisting of 754 amino acids has a calculated molecular mass of 85.7 kDa. As a result of glycosylation, the HA/mFc monomer migrates as an approximately 110-120 kDa protein in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.