Recombinant Influenza A virus (A/Shanghai/1/2013(H7N9)) hemagglutinin (Met1-Gly338), termed as HA1, was expressed with a C-terminal polyhistidine tag.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System.
The recombinant HA1 subnunit of Influenza A virus (A/Shanghai/1/2013(H7N9)) hemagglutinin comprises 331 amino acids and has a predicted molecular mass of 36.3 kDa. The apparent molecular mass of the protein is approximately 42 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.