Active Recombinant Influenza A H7N9 (A/Zhejiang/DTID-ZJU10/2013) HA protein(Met1-Val524), His-tagged

• Cat.No.: HA-746V
• Product Overview: Recombinant Influenza A H7N9 (A/Zhejiang/DTID-ZJU10/2013) HA protein(AHA11500.1)(Met1-Val524), fused with C-terminal His tag, was expressed in Insect Cells.

Specification

• similar: HA
• Alias: HA
• Source: Insect Cells
• Species: H7N9
• Conjugate: C-His
• Tag: C-His
• Protein length: Met1-Val524
• Form: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol,pH 8.0. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bioactivity: 1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglutinate guinea pig red blood cells. HA titer is 0.2-1 μg/mL for 1% GRBC.
• MolecularMass: The recombinant HA subunit of influenza A virus (A/Zhejiang/DTID-ZJU10/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa.
• Endotoxin: < 1.0 EU per μg protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• OfficialSymbol: HA