Recombinant influenza A virus (A/Zhejiang/DTID-ZJU10/2013(H7N9)) hemagglutinin (AHA11500.1) (Met1-Val524), termed as HA, was expressed with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol,pH 8.0, 5%~8% trehalose and mannitol.
1. Measured by its ability to bind with Neu5Aca2-3Galb1-4GlcNAcb-PAA-biotin (01-077) using the Octet RED System. 2. Measured by its ability to bind with Neu5Aca2-6GalNAca-PAA-biotin (01-059) using the Octet RED System. 3. Measured by its ability to agglut
The recombinant HA subunit of influenza A virus (A/Zhejiang/DTID-ZJU10/2013(H7N9)) comprises 517 amino acids and has a predicted molecular mass of 57.6 kDa.
< 1.0 EU per μg of the protein as determined by the LAL method.
>95 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.