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Recombinant HCoV S Protein, His-tagged

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Cat.No.:
S-563V
Product Overview:
Recombinant N-terminal fragment of human coronavirus (isolate HKU1) spike glycoprotein (YP_173238.1) (Met 1-Arg 760), corresponding to the S1 region, was fused with a polyhistidine tag at the C-terminus.
Source:
HEK293
Species:
HCoV
Tag:
His
Predicted N Terminal:
Gly 16
Form:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity:
Measured by its binding ability in a functional ELISA.Immobilized Spike-His (aa 1-760) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc with a linear range of 0.16-4.0 μg/ml.
Molecular Mass:
The secreted recombinant human coronavirus spike glycoprotein (aa 1-760) comprises 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>90 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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