Recombinant N-terminal fragment of human coronavirus (isolate HKU1) spike glycoprotein (YP_173238.1) (Met 1-Arg 760), corresponding to the S1 region, was fused with a polyhistidine tag at the C-terminus.
Predicted N Terminal:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Measured by its binding ability in a functional ELISA.Immobilized Spike-His (aa 1-760) at 10 μg/ml (100 μl/well) can bind biotinylated human ACE2-Fc with a linear range of 0.16-4.0 μg/ml.
The secreted recombinant human coronavirus spike glycoprotein (aa 1-760) comprises 756 amino acids with a predicted molecular mass of 85.8 kDa. As a result of high glycosylation, the apparent molecular mass of the recombinant protein is approximately 130-140 kDa in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>90 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.