Recombinant HIV gag Protein, His-tagged

• Cat.No.: gag-356V
• Product Overview: Recombinant amino acid (Pro 133-Leu 363) of HIV-1 gag protein (ACI05538.1), known as core nucleocapsid protein P24 was expressed, with a polyhistidine tag at the C-terminus and a Met at the N-terminus.

Specification

• Source: E. coli
• Species: HIV
• Tag: His
• Predicted N Terminal: Met 1
• Form: Lyophilized from sterile 0.14M PB, 0.5M NaCl, 20% glycerol, 1mM DTT, pH 8.0, 5%~8% trehalose and mannitol.
• Molecular Mass: The recombinant human HIV-1 Gag-p24 protein comprises 240 amino acids and has a predicted molecular mass of 26.5 kDa as estimated in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: >95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
• Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.