Address: 45-1 Ramsey Road, Shirley, NY 11967, USA    USA: 1-631-559-9269  1-631-448-7888   Fax: 1-631-938-8127  Europe: 44-207-048-3343   Email: info@creative-biomart.com
Home > Products > Recombinant Proteins > Recombinant HIV gp160 Protein, Fc-tagged

Recombinant HIV gp160 Protein, Fc-tagged

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"gp160" Related Products.
By Species
HIV(7)
By Source
E. coli(1)
HEK293(6)
By Tag
Fc(3)
His(3)
His & MBP(1)
Cat.No.:
gp160-330V
Product Overview:
Recombinant HIV-1 envelope glycoprotein gp160 extracellular domain (Ala 29-Ser 661), termed as gp140, was fused with the Fc region of human IgG1 at the C-terminus and a signal peptide at the N-terminus.
Source:
HEK293
Species:
HIV
Tag:
Fc
Predicted N Terminal:
Ala 29
Form:
Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity:
Using the Octet RED System, the affinity constant (Kd) of gp140-Fc bound to human CD4 is 0.3 μM.
Molecular Mass:
The recombinant HIV-1 gp140 consists of 874 amino acids after removal of the signal peptide and has a predicted molecular mass of 98 kDa. The apparent molecular mass of the recombinant protein is approximately 210-230 kDa in SDS-PAGE under reducing conditions.
Endotoxin:
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity:
>95 % as determined by SDS-PAGE.
Stability:
Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage:
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution:
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.


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Optional requirements on this protein +Expand
C-fusion   N-fusion  Non-tagged
His   GST  Fc  Others
<1.0 eu/μg   <0.1 eu/μg  <0.01 eu/μg  Not required
Monomer Isolation   Dimer Isolation   Not required
>80% by SDS-PAGE   >90% by SDS-PAGE  >95% by SDS-PAGE  Others
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45-1 Ramsey Road, Shirley, NY 11967, USA
USA: 1-631-559-9269  1-631-448-7888
Europe: 44-207-048-3343
FAX: 1-631-938-8127
EMAIL: info@creative-biomart.com

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