Recombinant HPV16 L1 was expressed in Hansenula polymorpha.
L1 played an important role in many functins.
20 mM Potassium Dihydrogen Phosphate (KH2PO4), pH 7.2 containing 320 mM Sodium Chloride, 1.7 mM EDTA, 0.01% Tween-80 and 10 mM histidine.
> 90% pure (SDS-PAGE). Purification from broken yeast cells by the following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size exclusion chromatography and sterile filtration.
Store at 2–8 centigrade. DO NOT FREEZE!
1.52 mg/mL (Lowry)