Recombinant HPV18 L1 was expressed in Hansenula polymorpha.
L1 played an important role in many functins.
20 mM Potassium Dihydrogen Phosphate (KH2PO4), pH 7.2 containing 500 mM Sodium Chloride, 1.7 mM EDTA, 0.01% Tween-80 and 1 mM 2-Mercaptoethanol.
> 90% pure (SDS-PAGE). Purification from broken yeast cells by the following scheme: clarification, ammonium sulfate precipitation, ultrafiltration, cation exchange chromatography, zonal centrifugation, size exclusion chromatography and filtration.
Store at 2–8 centigrade. DO NOT FREEZE!
2.22 mg/mL (Lowry)