Recombinant Hevea brasiliensis rq30 was expressed in E. coli.
Hev b 11-MBP is a recombinant fusion protein consisting of Maltose binding protein (MBP) and the 43 N-terminal AA of Hev b 11 fused to the C-terminal end of MBP. The construct contains a factor Xa cleaving site that allows the isolation of the Hev b 11 fragment.
lyophilized from 5 mM NH3HCO3 (volatile).
When stored at -20 centigrade the quality of the material will be maintained for several years. However, for short periods (max. 3 weeks) the lyophilized product may be kept at room temperature. After reconstitution store at -20 centigrade. Avoid repeated freezing/thawing.
The material should be reconstituted with distilled water (or equivalent) or dilute buffers. Do not use salt concentrations exceeding 20 mM to dissolve the lyophilized material. Salt may be added after dissolution. Gentle agitation during dissolution is essential, afterwards incubate for 30 min to allow a complete reconstitution of the protein. If reconstituted to 1 mg/ml the product will be soluble to at least 98%.