Recombinant Human ABCA1 protein, GST-tagged
Cat.No. : | ABCA1-178H |
Product Overview : | Recombinant Human ABCA1(86 a.a. - 185 a.a.) fused with GST tag at N-terminal was expressed in Wheat Germ. |
- Specification
- Gene Information
- Related Products
Description : | The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. With cholesterol as its substrate, this protein functions as a cholesteral efflux pump in the cellular lipid removal pathway. Mutations in this gene have been associated with Tangier's disease and familial high-density lipoprotein deficiency. |
Source : | Wheat Germ |
Species : | Human |
Tag : | GST |
Form : | 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Molecular Mass : | 36.74 kDa |
AA Sequence : | TPGEAPGVVGNFNKSIVARLFSDAR RLLLYSQKDTSMKDMRKVLRTLQQI KKSSSNLKLQDFLVDNETFSGFLYH NLSLPKSTVDKMLRADVILHKVFLQ |
Applications : | Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array |
Notes : | Best use within three months from the date of receipt of this protein. |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Gene Name : | ABCA1 ATP-binding cassette, sub-family A (ABC1), member 1 [ Homo sapiens ] |
Official Symbol : | ABCA1 |
Synonyms : | ABCA1; ATP-binding cassette, sub-family A (ABC1), member 1; ABC1, HDLDT1; ATP-binding cassette sub-family A member 1; Tangier disease; TGD; membrane-bound; ATP-binding cassette transporter 1; ATP-binding cassette transporter A1; cholesterol efflux regulatory protein; ABC1; CERP; ABC-1; HDLDT1; |
Gene ID : | 19 |
mRNA Refseq : | NM_005502 |
Protein Refseq : | NP_005493 |
UniProt ID : | O95477 |
Chromosome Location : | 9q31 |
Pathway : | ABC transporters, organism-specific biosystem; ABC transporters, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fatty acid, triacylglycerol, and ketone body metabolism, organism-specific biosystem; Folate Metabolism, organism-specific biosystem; HDL-mediated lipid transport, organism-specific biosystem; |
Function : | ATP binding; ATPase activity; anion transmembrane transporter activity; apolipoprotein A-I binding; apolipoprotein A-I receptor activity; apolipoprotein binding; cholesterol binding; cholesterol transporter activity; nucleotide binding; phospholipid binding; phospholipid transporter activity; protein binding; small GTPase binding; syntaxin binding; |
Products Types
◆ Recombinant Protein | ||
ABCA1-2493H | Recombinant Human ABCA1 protein(1171-1280 aa), C-His-tagged | +Inquiry |
ABCA1-2398H | Recombinant Human ABCA1 Protein, His (Fc)-Avi-tagged | +Inquiry |
ABCA1-10H | Recombinant Human ABCA1 Protein, His-tagged | +Inquiry |
ABCA1-0286H | Recombinant Human ABCA1 Protein (A2-V2261), Flag, Strep II, 8×His tagged | +Inquiry |
ABCA1-3140H | Recombinant Human ABCA1 protein, His-tagged | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a questionWe have multiple protein expression platforms, including baculovirus-insect cell expression systems, bacterial expression systems (E. coli / Bacillus), mammalian expression systems, yeast expression systems (P pastoris / S cerevisiae), Nicotiana tabacum specific expression platform.
You can try to optimize the protocol for preparing stacking gel and running gel. Appropriately prolonging the running gel polymerization time and completely removing water before pouring into the stacking gel are directions that can be optimized.
Both trehalose and mannitol are added as lyoprotectants, which can significantly prevent protein structure changes, elongation and aggregation during freeze-drying.
Removing labels may affect product stability. If you really need to remove the label, please contact us, we will customize the recombinant protein product according to your needs.
Generally, recombinant proteins expressed by prokaryotic cells are in the state of lyophilized powder and need to be dissolved before use. However, the recombinant protein expressed by eukaryotic cells is more conducive to maintaining its activity and stability when stored in a solution state.
ABCA1 protein is mainly located in the cell membrane.
You can try to use 8%PAGE.
ABCA1 is a membrane protein, and it is necessary to avoid protein damage during sample processing. You may need to further optimize the sample handling process.
You can contact our technical experts directly by email or phone, and they will give you relevant advice.
It is recommended to check whether the antibody is suitable, including the specificity and concentration settings.
Customer Reviews (7)
Write a reviewWork great in Western blot analysis.
The after-sales service is in place, able to reply to my questions in time and help me solve the problems in the experiment.
Great recombinant protein product. The instruction manual attached to the product is detailed and provided me with some help.
Useful in structure analysis.
I purchased ABCA1 protein as a positive reference for western blot and got clear and distinct bands.
Good
The technical experts answered my doubts and let me choose the one suitable for my research from a variety of ABCA1 products.
Ask a Question for All ABCA1 Products
Required fields are marked with *
My Review for All ABCA1 Products
Required fields are marked with *
Inquiry Basket