Recombinant Human ACAT2 protein, T7-tagged
Cat.No. : | ACAT2-166H |
Product Overview : | Recombinant human ACAT2 fused with T7 Tag at N-terminal was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Source : | E. coli |
Species : | Human |
Tag : | T7 |
Form : | 1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT. |
AA Sequence : | MASMTGGQQMGRGEFGSNAGSDPVV IVSAARTIIGSFNGALAAVPVQDLG STVIKEVLKRATVAPEDVSEVIFGH VLAAGCGQNPVRQASVGAGIPYSVP AWSCQMICGSGLKAVCLAVQSIGIG DSSIVVAGGMENMSKAPHLAYLRTG VKIGEMPLTDSILCDGLTDAFHNCH MGITAENVAKKWQVSREDQDKVAVL SQNRTENAQKAGHFDKEIVPVLVST RKGLIEVKTDEFPRHGSNIEAMSKL KPYFLTDGTGTVTPANASGINDGAA AVVLMKKSEADKRGLTPLARIVSWS QVGVEPSIMGIGPIPAIKQAVTKAG WSLEDVDIFEINEAFAAVSAAIVKE LGLNPEKVNIEGGAIALGHPLGASG CRILVTLLHTLERMGRSRGVAALCI GGGMGIAMCVQRE |
Purity : | >90% by SDS-PAGE |
Applications : | 1. May be used for cell lipid metabolic pathway regulation study in vitro using recombinant ACAT2 protein intracellular delivery methods.2. May be used for in vitro protein–protein interaction measurement for mapping ACAT2 binder.3. Excellent lysine acetylation subtract protein for enzymatic assay development.4. May be used as antigen for specific antibody development. |
Storage : | Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days. |
Gene Name : | ACAT2 acetyl-CoA acetyltransferase 2 [ Homo sapiens ] |
Official Symbol : | ACAT2 |
Synonyms : | ACAT2; acetyl-CoA acetyltransferase 2; acetyl Coenzyme A acetyltransferase 2 , acetyl Coenzyme A acetyltransferase 2 (acetoacetyl Coenzyme A thiolase); acetyl-CoA acetyltransferase, cytosolic; acetoacetyl Coenzyme A thiolase; cytosolic acetoacetyl-CoA thiolase; acetyl-CoA transferase-like protein; |
Gene ID : | 39 |
mRNA Refseq : | NM_005891 |
Protein Refseq : | NP_005882 |
MIM : | 100678 |
UniProt ID : | Q9BWD1 |
Chromosome Location : | 6q25.3-q26 |
Pathway : | Butanoate metabolism, organism-specific biosystem; Butanoate metabolism, conserved biosystem; C5 isoprenoid biosynthesis, mevalonate pathway, organism-specific biosystem; C5 isoprenoid biosynthesis, mevalonate pathway, conserved biosystem; Fat digestion and absorption, organism-specific biosystem; Fat digestion and absorption, conserved biosystem; Fatty acid metabolism, organism-specific biosystem; |
Function : | acetyl-CoA C-acetyltransferase activity; transferase activity, transferring acyl groups other than amino-acyl groups; |
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◆ Lysates | ||
ACAT2-9108HCL | Recombinant Human ACAT2 293 Cell Lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionTypically, protein purity is measured using techniques such as SDS-PAGE, Western blotting, or HPLC.
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Creative BioMart uses a variety of quality control measures to ensure the purity, identity, and activity of our products. These may include analytical testing such as SDS-PAGE, Western blotting, and ELISA, as well as functional assays to confirm activity. We can also perform endotoxin testing or use other methods to ensure the product is free from contaminants.
Like all proteins, recombinant ACAT2 protein should be handled with appropriate safety precautions, such as wearing gloves and avoiding direct contact with skin or mucous membranes. It is also important to follow good laboratory practices and to dispose of any waste materials properly.
The recommended concentration and volume of recombinant ACAT2 protein can vary depending on the specific assay or experiment being performed. Typically, the protein is dissolved in a buffer solution at a desired concentration (e.g. 0.1-1 mg/mL) and then used as needed. It is important to ensure that the protein concentration is appropriate for the intended use, as high concentrations may result in nonspecific binding or other issues.
Recombinant ACAT2 protein can be used in a variety of research applications, including studying the function of ACAT2 in lipid metabolism and cholesterol homeostasis, as well as in drug discovery efforts related to cardiovascular disease, obesity, and other metabolic disorders. It may also be used as a reference or standard in diagnostic tests for certain diseases.
The expression and activity of ACAT2 are regulated by a variety of factors, including dietary cholesterol intake, hormonal signals, and transcriptional and post-transcriptional mechanisms. Studies have identified several transcription factors that bind to the ACAT2 promoter region and regulate its expression, as well as post-transcriptional mechanisms such as alternative splicing and miRNA-mediated regulation.
In addition to recombinant ACAT2 protein, there may be natural sources of ACAT2 protein, such as from animal or human tissues. However, these sources may be more difficult to obtain and may have variable purity or activity. It is important to evaluate the quality and activity of these alternative sources before use in experiments or assays.
Inhibition of ACAT2 has been proposed as a potential therapeutic strategy for the treatment of cardiovascular disease, as well as other metabolic disorders such as nonalcoholic fatty liver disease (NAFLD) and type 2 diabetes. Several small molecule inhibitors of ACAT2 have been developed and are currently undergoing preclinical and clinical evaluation.
ACAT2 is a key enzyme involved in the conversion of free cholesterol to cholesterol esters, which are stored in lipid droplets in cells. Dysregulation of ACAT2 activity has been implicated in the development of atherosclerosis and other metabolic disorders. Studies have shown that inhibition of ACAT2 can reduce atherosclerotic plaque formation in animal models, suggesting that ACAT2 may be a potential therapeutic target for the prevention and treatment of cardiovascular disease.
Customer Reviews (3)
Write a reviewThe purity and quality of Creative BioMart's recombinant human ACAT2 protein is unmatched. I have tried other protein products in the past, but this one is by far the best.
The recombinant human ACAT2 protein is of high quality and purity, and it has worked perfectly in my experiments.
The recombinant human ACAT2 protein from Creative BioMart is highly pure and stable, and I have had great success using it in my assays.
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