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Recombinant Human ADA Protein, 1-363aa, N-His tagged

Cat.No. : ADA-40H
Product Overview : Recombinant human ADA protein (1-363aa), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.
  • Specification
  • Gene Information
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Description : This gene encodes an enzyme that catalyzes the hydrolysis of adenosine to inosine in the purine catabolic pathway. Various mutations have been described for this gene and have been linked to human diseases related to impaired immune function such as severe combined immunodeficiency disease (SCID) which is the result of a deficiency in the ADA enzyme. In ADA-deficient individuals there is a marked depletion of T, B, and NK lymphocytes, and consequently, a lack of both humoral and cellular immunity. Conversely, elevated levels of this enzyme are associated with congenital hemolytic anemia.
Source : E. coli
Species : Human
Tag : N-His
Protein length : 1-363aa
Form : Liquid
Bio-activity : > 40 unit/mg, and is defined as the amount of enzyme that convert 1.0 μmol of adenosine to inosine per minute at pH 7.5 at 25 centigrade.
Molecular Mass : 42.9 kDa (383aa) confirmed by MALDI-TOF
AA Sequence : MAQTPAFDKPKVELHVHLDGSIKPE TILYYGRRRGIALPANTAEGLLNVI GMDKPLTLPDFLAKFDYYMPAIAGC REAIKRIAYEFVEMKAKEGVVYVEV RYSPHLLANSKVEPIPWNQAEGDLT PDEVVALVGQGLQEGERDFGVKARS ILCCMRHQPNWSPKVVELCKKYQQQ TVVAIDLAGDETIPGSSLLPGHVQA YQEAVKSGIHRTVHAGEVGSAEVVK EAVDILKTERLGHGYHTLEDQALYN RLRQENMHFEICPWSSYLTGAWKPD TEHAVIRLKNDQANYSLNTDDPLIF KSTLDTDYQMTKRDMGFTEEEFKRL NINAAKSSFLPEDEKRELLDLLYKA YGMPPSASAGQNL
Endotoxin : < 1 EU/μg of protein (determined by LAL method)
Purity : > 90% by SDS-PAGE
Applications : SDS-PAGE, Enzyme Activity
Storage : Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
Concentration : 0.5 mg/mL (determined by Bradford assay)
Storage Buffer : 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
References : 1. Gines S., et al. (2002) Biochem. 361:203-209
2. Daddona P.E., et al. (1984) J. Biol. Chem. 259:12101-12106
Gene Name : ADA adenosine deaminase [ Homo sapiens (human) ]
Official Symbol : ADA
Synonyms : ADA; adenosine deaminase; ADA1; adenosine deaminase; adenosine aminohydrolase; EC 3.5.4.4
Gene ID : 100
mRNA Refseq : NM_000022
Protein Refseq : NP_000013
MIM : 608958
UniProt ID : P00813

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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