Recombinant Human ADIPOQ(Glu 19 - Asn 244) fused with His tag at the C-terminus was expressed in HEK293.
Predicted N Terminal:
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally Mannitol or Trehalose are added as protectants before lyophilization.
The ED50 for this effect is typically 2 - 10 µg/ml.
Has a calculated MW of 25.4 kDa. DTT-reduced Protein migrates as 28-33 kDa due to glycosylation.
Glu 19 - Asn 244
Less than 1.0 EU per μg of the rhADIPOQ / ACRP30 by the LAL method.
>92% as determined by SDS-PAGE.
Avoid repeated freeze-thaw cycles.No activity loss was observed after storage at:In lyophilized state for 1 year (4 centigrade-8 centigrade); After reconstitution under sterile conditions for 1 month (4 centigrade-8 centigrade) or 3 months (-20 centigrade to -70 centigrade).