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Recombinant Human AFF1 Protein, GST-tagged

Cat.No. : AFF1-405H
Product Overview : Human AFF1 partial ORF ( NP_005926.1, 71 a.a. - 170 a.a.) recombinant protein with GST-tag at N-terminal.
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Description : This gene encodes a member of the AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome family of proteins, which have been implicated in childhood lymphoblastic leukemia, Fragile X E site mental retardation, and ataxia. It is the prevalent mixed-lineage leukemia fusion gene associated with spontaneous acute lymphoblastic leukemia. Members of this family have three conserved domains: an N-terminal homology domain, an AF4/ lymphoid nuclear protein related to AF4/Fragile X E mental retardation syndrome domain, and a C-terminal homology domain. The protein functions as a regulator of RNA polymerase II-mediated transcription through elongation and chromatin remodeling functions. Through RNA interference screens, this gene has been shown to promote the expression of CD133, a plasma membrane glycoprotein required for leukemia cell survival. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 36.74 kDa
AA Sequence : LSTKSHTHRLDASENRLGKPKYPLI PDKGSSIPSSSFHTSVHHQSIHTPA SGPLSVGNISHNPKMAQPRTEPMPS LHAKSCGPPDSQHLTQDRLGQEGFG
Applications : Enzyme-linked Immunoabsorbent Assay
Western Blot (Recombinant protein)
Antibody Production
Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : AFF1 AF4/FMR2 family, member 1 [ Homo sapiens ]
Official Symbol : AFF1
Synonyms : AFF1; AF4/FMR2 family, member 1; MLLT2, myeloid/lymphoid or mixed lineage leukemia (trithorax (Drosophila) homolog); translocated to, 2, PBM1, pre B cell monocytic leukemia partner 1; AF4/FMR2 family member 1; AF 4; AF4; protein AF-4; proto-oncogene AF4; pre-B-cell monocytic leukemia partner 1; ALL1-fused gene from chromosome 4 protein; myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila); translocated to, 2; PBM1; MLLT2; MGC134969;
Gene ID : 4299
mRNA Refseq : NM_001166693
Protein Refseq : NP_001160165
MIM : 159557
UniProt ID : P51825

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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Can AFF1 protein be targeted or manipulated to modulate gene expression or disease phenotypes, and what are the potential implications for therapeutic interventions? 11/07/2021

AFF1 protein holds potential as a target for modulating gene expression or disease phenotypes, and further research is needed to explore its implications for therapeutic interventions.

Are there any genetic variations or mutations in the gene encoding AFF1 protein that impact its expression or function, and what are the implications for cellular processes or disease susceptibility? 05/11/2021

Genetic variations or mutations in the gene encoding AFF1 protein may impact its expression or function, influencing cellular processes or disease susceptibility.

How does AFF1 protein contribute to cellular processes such as development, differentiation, or disease progression, and what are the underlying molecular mechanisms? 10/08/2020

AFF1 protein contributes to cellular processes such as development, differentiation, or disease progression, and the underlying molecular mechanisms are currently being investigated.

What experimental techniques or assays have been used to study the functional significance of AFF1 protein, such as chromatin immunoprecipitation, RNA-seq, or gene knockdown approaches? 05/23/2018

Various experimental techniques or assays, such as chromatin immunoprecipitation, RNA-seq, or gene knockdown approaches, have been used to study the functional significance of AFF1 protein.

Are there any known post-translational modifications or regulatory mechanisms that modulate the activity or stability of AFF1 protein? 07/05/2017

Post-translational modifications and regulatory mechanisms may modulate the activity or stability of AFF1 protein.

How does dysregulation or dysfunction of AFF1 protein affect transcriptional regulation, gene expression networks, or disease development? 03/22/2017

Dysregulation or dysfunction of AFF1 protein can affect transcriptional regulation, perturb gene expression networks, and contribute to disease development.

How is the expression of AFF1 protein regulated, and what factors or signaling pathways control its expression? 08/16/2015

The expression of AFF1 protein is regulated by specific factors and signaling pathways.

What is the role of AFF1 protein in gene transcription or chromatin remodeling, and how can its function be investigated experimentally? 05/02/2015

AFF1 protein plays a role in gene transcription or chromatin remodeling, and its function can be investigated using techniques like chromatin immunoprecipitation or gene expression analysis.

What is the subcellular localization of AFF1 protein, and how can it be experimentally determined? 04/25/2015

The subcellular localization of AFF1 protein can be experimentally determined using techniques such as immunofluorescence microscopy or subcellular fractionation.

Does AFF1 protein interact with specific molecules or participate in protein complexes, and what are the functional implications of these interactions? 04/12/2015

AFF1 protein may interact with specific molecules or participate in protein complexes, which have functional implications in cellular processes.

Customer Reviews (3)

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Reviews
12/15/2021

    Investigating protein-protein interactions in cellular senescence for aging-related diseases.

    04/07/2019

      Mapping protein-protein interactions in immune checkpoint regulation for immunotherapy.

      03/18/2017

        Unraveling protein-protein interactions in RNA splicing for gene expression regulation.

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