Recombinant Human AFP fusion protein was constructed by fusion human alpha fetal protein (AFP) N-terminal domain-I (1-198 aa) with human collagen type I derived AAQIRSQVMTHLRVIYER peptide and was expressed in E. coli.
Cell adhesion to the extracellular matrix (ECM) is essential for the development and maintenance of cells. Cell adhesion to various ECM as ligands is mainly mediated by cell surface receptor such as integrins. Human Collagen Type I is a key component in ECM for cell attachment and differentiation. M2 Peptide AAQIRSQVMTHLRVIYER that derived from human collagen type I protein has been demonstrated in vitro as collagen mimetics and specific interacts with APP. This peptide will be a good coating matrix for cell attachment in serum free medium or enhance specific cell lineage differentiation.
1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
1. As fusion protein fully derived from human protein, can be used as coating matrix protein to replace human collagen type I for human cell differentiation regulation study in vitro.2. Can be used in low serum or serum-free cell culture media to improve attachment and spreading of many normal and stem cells including human ES cell, endothelial, epithelial, hepatocytes and mesenchymal stem cells.3. Can be used to improve survival of primary cultures.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.