Recombinant Human ALDH4A1 Protein, His & GST-tagged
Recombinant mature form of human ALDH4A1 (AAH07581.1) (Lys 25-Gln 563) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.5, 10% glycerol, 5%~8% trehalose and mannitol.
The recombinant human ALDH4A1/GST chimera consists of 776 amino acids and has a calculated molecular mass of 86.8 KDa. It migrates as an approximately 75 KDa band in SDS-PAGE under reducing conditions.
< 1.0 EU per μg of the protein as determined by the LAL method.
>96 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.