Recombinant Human ALK, cytoplasmic domain [1058-1620(end) amino acids and L1196M of accession numberBAG10812.1] fused with DYKDDDDK tag at N-terminal was expressed in insect cell and biotinylated.
50 mM Tris-HCl, 150 mM NaCl, 0.05% Brij35, 1 mM DTT, 10% glycerol, pH7.5
>85% as determined by SDS-PAGE and CBB staining
Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.