Recombinant Human ALPL Protein, GST-tagged
Cat.No. : | ALPL-494H |
Product Overview : | Human ALPL full-length ORF ( NP_000469.3, 1 a.a. - 524 a.a.) recombinant protein with GST-tag at N-terminal. |
- Specification
- Gene Information
- Related Products
Description : | This gene encodes a member of the alkaline phosphatase family of proteins. There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney (tissue non-specific). The first three are located together on chromosome 2, while the tissue non-specific form is located on chromosome 1. The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature enzyme. This enzyme may play a role in bone mineralization. Mutations in this gene have been linked to hypophosphatasia, a disorder that is characterized by hypercalcemia and skeletal defects. [provided by RefSeq, Oct 2015] |
Source : | Wheat Germ |
Species : | Human |
Tag : | GST |
Molecular Mass : | 83.7 kDa |
AA Sequence : | MISPFLVLAIGTCLTNSLVPEKEKD PKYWRDQAQETLKYALELQKLNTNV AKNVIMFLGDGMGVSTVTAARILKG QLHHNPGEETRLEMDKFPFVALSKT YNTNAQVPDSAGTATAYLCGVKANE GTVGVSAATERSRCNTTQGNEVTSI LRWAKDAGKSVGIVTTTRVNHATPS AAYAHSADRDWYSDNEMPPEALSQG CKDIAYQLMHNIRDIDVIMGGGRKY MYPKNKTDVEYESDEKARGTRLDGL DLVDTWKSFKPRYKHSHFIWNRTEL LTLDPHNVDYLLGLFEPGDMQYELN RNNVTDPSLSEMVVVAIQILRKNPK GFFLLVEGGRIDHGHHEGKAKQALH EAVEMDRAIGQAGSLTSSEDTLTVV TADHSHVFTFGGYTPRGNSIFGLAP MLSDTDKKPFTAILYGNGPGYKVVG GERENVSMVDYAHNNYQAQSAVPLR HETHGGEDVAVFSKGPMAHLLHGVH EQNYVPHVMAYAACIGANLGHCAPA SSAGSLAAGPLLLALALYPLSVLF |
Applications : | Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array |
Notes : | Best use within three months from the date of receipt of this protein. |
Storage : | Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. |
Storage Buffer : | 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer. |
Gene Name : | ALPL alkaline phosphatase, liver/bone/kidney [ Homo sapiens ] |
Official Symbol : | ALPL |
Synonyms : | ALPL; alkaline phosphatase, liver/bone/kidney; HOPS; alkaline phosphatase, tissue-nonspecific isozyme; TNSALP; glycerophosphatase; tissue-nonspecific ALP; alkaline phosphomonoesterase; liver/bone/kidney-type alkaline phosphatase; alkaline phosphatase liver/bone/kidney isozyme; TNAP; APTNAP; AP-TNAP; FLJ40094; FLJ93059; MGC161443; MGC167935; |
Gene ID : | 249 |
mRNA Refseq : | NM_000478 |
Protein Refseq : | NP_000469 |
MIM : | 171760 |
UniProt ID : | P05186 |
Products Types
◆ Recombinant Protein | ||
ALPL-1994H | Recombinant Human ALPL Protein, MYC/DDK-tagged | +Inquiry |
ALPL-1299M | Recombinant Mouse ALPL Protein (20-503 aa), His-tagged | +Inquiry |
Alpl-487M | Recombinant Mouse Alpl Protein, His (Fc)-Avi-tagged | +Inquiry |
ALPL-028H | Recombinant Human ALPL Protein, MYC/DDK-tagged, C13 and N15-labeled | +Inquiry |
ALPL-297R | Recombinant Rat ALPL Protein, His (Fc)-Avi-tagged | +Inquiry |
◆ Native Protein | ||
ALPL-25H | Active Native Human ALPL Protein | +Inquiry |
ALPL-8004H | Native Human Liver Alkaline Phosphatase | +Inquiry |
ALPL-66C | Active Native Calf Alkaline Phosphatase | +Inquiry |
◆ Lysates | ||
ALPL-3092HCL | Recombinant Human ALPL cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
Inquiry
- Q&As
- Reviews
Q&As (10)
Ask a questionALPL exhibits tissue-specific expression, with high levels in mineralizing tissues such as bones and teeth, but lower levels in other organs.
ALPL activity and expression undergo dynamic changes during development and aging, reflecting the physiological demands for mineralization.
ALPL activity can be modulated by factors such as pyrophosphate, calcium, and inhibitors like levamisole, which impact mineralization outcomes.
ALPL plays a critical role in mineralization processes by generating inorganic phosphate, an essential component of hydroxyapatite crystals in bones and teeth.
ALPL can serve as a diagnostic marker for hypophosphatasia and related mineralization disorders, and therapeutic strategies targeting ALPL are being explored.
ALPL deficiency or dysregulation is associated with hypophosphatasia, a genetic disorder characterized by impaired mineralization and skeletal abnormalities.
Genetic variations or mutations in the ALPL gene can lead to altered enzyme activity, affecting mineralization and contributing to disease phenotypes.
Manipulating ALPL activity and function holds promise for promoting bone regeneration and dental tissue engineering, enhancing mineralization processes and tissue formation.
ALPL regulates molecular interactions and signaling pathways, including Wnt/尾-catenin, BMP, and FGF signaling, to orchestrate mineralization processes.
ALPL gene expression is regulated by transcription factors, epigenetic modifications, and signaling pathways involved in skeletal development and mineralization.
Customer Reviews (3)
Write a reviewEfficient target protein purification.
Reliable antibody for Western blotting.
Robust enzyme activity measurement.
Ask a Question for All ALPL Products
Required fields are marked with *
My Review for All ALPL Products
Required fields are marked with *
Inquiry Basket