Recombinant human ALPP cDNA (23-506aa, derived from BC009647) fused with T7-His-TEV cleavage site Tag at N-terminal was expressed in E. coli.
1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFIIPVEEENPDFWNREAAEALGAAKKLQPAQTAAKNLIIFLGDGMGV STVTAARILKGQKKDKLGPEIPLAMDRFPYVALSKTYNVDKHVPDSGATATAYLCGVKGNFQTIGLSAAARFNQC NTTRGNEVISVMNRAKKAGKSVGVVTTTRVQHASPAGTYAHTVNRNWYSDADVPASARQEGCQDIATQLISNMDI DVILGGGRKYMFRMGTPDPEYPDDYSQGGTRLDGKNLVQEWLAKRQGARYVWNRTELMQASLDPSVTHLMGLFEP GDMKYEIHRDSTLDPSLMEMTEAALRLLSRNPRGFFLFVEGGRIDHGHHESRAYRALTETIMFDDAIERAGQLTS EEDTLSLVTADHSHVFSFGGYPLRGSSIFGLAPGKARDRKAYTVLLYGNGPGYVLKDGARPDVTESESGSPEYRQ QSAVPLDEETHAGEDVAVFARGPQAHLVHGVQEQTFIAHVMAFAACLEPYTACDLAPPAGTTD
1. May be used for in vitro ALPP mediated Insulin-like growth factor (IGF) pathway regulation study in stem cell proliferation by coating this protein as matrix protein or use it as soluble factor.2. May be used for protein-protein interaction mapping.3. As enzymatic substrate for various proteases.4. Potential biomarker protein for tumor diagnosis, such as ovarian cancer, et al.5. As immunogen for specific antibody production.
Keep at -80 centigrade for long term storage. Product is stable at 4 centigrade for at least 7 days.