Recombinant human ANGPTL4 (Q9BY76) (Pro166-Ser406) was expressed,with the fused Fc region of human IgG1 at the N-terminus.
Predicted N Terminal:
Lyophilized from sterile 50mM Tris-Citrate, 0.3 M NaCl, pH 6.5, 5%~8% trehalose and mannitol.
The recombinant human ANGPTL4/Fc is a disulfide-linked homodimer. The reduced monomer comprises 501 amino acids and has a predicted molecular mass of 55.5 kDa. The apparent molecular mass of the protein is approximately 65 kDa in SDS-PAGE under reducing conditions due to glycosylation.
< 1.0 EU per μg of the protein as determined by the LAL method.
>92 % as determined by SDS-PAGE.
Samples are stable for up to twelve months from date of receipt at -70ºC.
Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.