Recombinant Human ANXA1(Met1-Asn346) fused with His tag at C-terminal was expressed in E. coli.
Predicted N Terminal:
Lyophilized from a 0.2 μm filtered solution in PBS.
Measured by its ability to inhibit proliferation of A549 human lung carcinoma cells. The ED50 for this effect is typically 1.0-4.0 μg/mL.
Predicted Molecular Mass: 39.4 kDa;SDS-PAGE: 38 kDa, reducing conditions.
<0.01 EU per 1 μg of the protein by the LAL method.
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Avoid repeated freeze-thaw cycles.12 months from date of receipt, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitute at 400 μg/mL in PBS.