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Recombinant Human ARF6 protein, GST-tagged

Cat.No. : ARF6-749H
Product Overview : Human ARF6 full-length ORF ( AAH08918, 1 a.a. - 175 a.a.) recombinant protein with GST-tag at N-terminal.
  • Specification
  • Gene Information
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Description : This gene encodes a member of the human ARF gene family, which is part of the RAS superfamily. The ARF genes encode small guanine nucleotide-binding proteins that stimulate the ADP-ribosyltransferase activity of cholera toxin and play a role in vesicular trafficking and as activators of phospholipase D. The product of this gene is localized to the plasma membrane, and regulates vesicular trafficking, remodelling of membrane lipids, and signaling pathways that lead to actin remodeling. A pseudogene of this gene is located on chromosome 7. [provided by RefSeq, Jul 2008]
Source : Wheat Germ
Species : Human
Tag : GST
Molecular Mass : 44.99 kDa
AA Sequence : MGKVLSKIFGNKEMRILMLGLDAAG KTTILYKLKLGQSVTTIPTVGFNVE TVTYKNVKFNVWDVGGQDKIRPLWR HYYTGTQGLIFVVDCADRDRIDEAR QELHRIINDREMRDAIILIFANKQD LPDAMKPHEIQEKLGLTRIRDRNWY VQPSCATSGDGLYEGLTWLTSNYKS
Applications : Enzyme-linked Immunoabsorbent Assay; Western Blot (Recombinant protein); Antibody Production; Protein Array
Notes : Best use within three months from the date of receipt of this protein.
Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing.
Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.
Gene Name : ARF6 ADP-ribosylation factor 6 [ Homo sapiens ]
Official Symbol : ARF6
Synonyms : ARF6; ADP-ribosylation factor 6; DKFZp564M0264;
Gene ID : 382
mRNA Refseq : NM_001663
Protein Refseq : NP_001654
MIM : 600464
UniProt ID : P62330

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (23)

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Can ARF6 regulate recycling of internalized membrane proteins? 03/13/2023

Yes, ARF6 is involved in the recycling of internalized membrane proteins. After macropinocytosis or endocytosis, ARF6 directs the trafficking of internalized proteins back to the plasma membrane through a recycling pathway, maintaining cellular homeostasis and regulating membrane protein levels.

How is ARF6 involved in cancer metastasis? 01/19/2023

ARF6 is implicated in cancer metastasis, the spread of cancer cells to distant organs. It promotes cancer cell invasion by regulating actin dynamics and membrane protrusions, facilitating tumor cell migration and invasion through the extracellular matrix. ARF6 dysregulation can enhance the invasive properties of cancer cells.

Can dysregulation of ARF6 contribute to neurodegenerative diseases? 11/28/2022

Yes, dysregulation of ARF6 has been implicated in neurodegenerative diseases. For example, in Alzheimer's disease, ARF6 dysregulation has been associated with the accumulation of amyloid-beta plaques and the formation of neurofibrillary tangles, which are hallmark features of the disease. ARF6 is also involved in the regulation of lysosomal function and clearance of protein aggregates, and dysfunction in these processes can contribute to neurodegeneration. Similarly, in Parkinson's disease, ARF6 dysregulation has been linked to the accumulation of alpha-synuclein protein aggregates and the impairment of mitochondrial function, which are key pathological events in the disease.

Are there specific inhibitors or activators for ARF6? 11/04/2022

While there are no specific inhibitors or activators for ARF6 that are widely available, certain compounds like SecinH3 and brefeldin A have been shown to inhibit ARF6 activation indirectly by targeting its upstream regulators, GEFs.

Can targeting ARF6 be a potential therapeutic strategy for certain diseases? 07/22/2022

Targeting ARF6 could be a potential therapeutic strategy for diseases in which dysregulated membrane trafficking, actin dynamics, or cell adhesion plays a critical role. However, more research is needed to understand the precise mechanisms and consequences of modulating ARF6 activity before considering it as a specific therapeutic target.

Can ARF6 be modified by post-translational modifications? 01/12/2022

Yes, ARF6 can undergo post-translational modifications, including phosphorylation, acetylation, and ubiquitination. These modifications can regulate ARF6's activity, localization, and interactions with other proteins.

Can ARF6 be involved in insulin signaling and glucose metabolism? 02/19/2021

Yes, ARF6 has been linked to insulin signaling and glucose metabolism. It plays a role in the trafficking of glucose transporter 4 (GLUT4), a critical protein involved in insulin-mediated glucose uptake in muscle and fat cells. Dysregulation of ARF6 activity can affect GLUT4 trafficking, impairing insulin responsiveness and contributing to insulin resistance.

Is ARF6 involved in inflammation and immune responses? 10/01/2020

Yes, ARF6 is involved in inflammation and immune responses. It participates in the regulation of immune cell trafficking, antigen presentation, and phagocytosis. ARF6 also plays a role in the release of inflammatory mediators and cytokines, contributing to the immune response in various conditions, such as infections and autoimmune diseases.

Is ARF6 associated with any human diseases? 04/10/2020

Dysregulation of ARF6 has been implicated in various diseases. It is involved in cancer progression, including metastasis, as well as neurodegenerative disorders, inflammatory diseases, and infectious diseases. ARF6 dysfunctions can lead to altered membrane trafficking, cell signaling, and cytoskeletal dynamics, contributing to disease pathogenesis.

Can ARF6 play a role in endocytosis and membrane trafficking? 03/29/2020

Yes, ARF6 plays a crucial role in endocytosis and membrane trafficking. It regulates the internalization and recycling of various cell surface receptors and transporters. ARF6 also controls the trafficking of intracellular compartments, such as endosomes and lysosomes. Dysregulation of ARF6 can disrupt these processes, leading to altered receptor signaling, impaired protein sorting, and abnormal membrane dynamics.

Can ARF6 be regulated by cellular stress or environmental cues? 08/24/2019

Yes, ARF6 activity can be regulated by cellular stress and environmental cues. For example, oxidative stress can activate ARF6, leading to increased endocytosis and altered cellular responses. Similarly, various extracellular signals, such as growth factors or chemotactic factors, can modulate ARF6 activity to regulate cellular responses.

Are there any known downstream effectors or binding partners of ARF6? 08/06/2019

ARF6 interacts with various downstream effectors and binding partners. These include cytoskeletal regulators like WAVE complex and WASP, membrane trafficking proteins like EFA6 and ARNO, as well as signaling molecules such as phospholipase D and Rac1.

Are there any drugs or therapeutic approaches targeting ARF6? 02/02/2019

Currently, there are no specific drugs targeting ARF6 that are approved for clinical use. However, the development of small molecules or biologics that selectively modulate ARF6 activity is an active area of research. Several compounds, such as SecinH3, have been identified as potential inhibitors of ARF6 activation and are being explored for their therapeutic potential in diseases where ARF6 dysregulation is implicated.

Can ARF6 be a potential therapeutic target in cancer treatment? 10/27/2018

Yes, ARF6 is being explored as a potential therapeutic target in cancer treatment. Its involvement in tumor cell invasion and metastasis makes it an attractive candidate for intervention. Modulating ARF6 activity or targeting its downstream effectors may help inhibit cancer cell migration, invasion, and metastasis. However, further research is needed to fully understand the therapeutic potential and feasibility of targeting ARF6 in cancer treatment.

Does ARF6 have a role in neuronal development and synaptic function? 07/31/2018

Yes, ARF6 plays a role in neuronal development and synaptic function. It is involved in dendritic spine formation, axonal growth, and synaptic vesicle recycling. ARF6 regulates the trafficking of synaptic proteins and neurotransmitter receptors, thus modulating synaptic transmission and plasticity.

Are there any known mutations in ARF6 associated with genetic disorders? 04/18/2017

Mutations in ARF6 have been identified in certain genetic disorders. For example, a missense mutation in ARF6 has been found in individuals with periventricular heterotopia, a condition characterized by the abnormal migration of neurons in the developing brain. Other studies have identified ARF6 mutations in patients with intellectual disability and developmental delays. These findings suggest a role for ARF6 in neurodevelopmental disorders.

Does ARF6 have a role in cell migration and invasion? 10/23/2016

Yes, ARF6 plays a critical role in cell migration and invasion. It regulates processes such as lamellipodia formation, focal adhesion turnover, and invasion through extracellular matrix. Dysregulation of ARF6 can contribute to enhanced migration and invasion abilities of cancer cells.

Is ARF6 involved in cell proliferation and cell cycle regulation? 10/03/2016

Yes, ARF6 is involved in cell proliferation and cell cycle regulation. It has been shown to modulate cell cycle progression by regulating the expression and activity of cyclin-dependent kinases (CDKs) and cyclins. ARF6 can also influence cell proliferation through its interactions with growth factor receptors and downstream signaling pathways.

Can ARF6 regulate actin cytoskeleton dynamics? 07/04/2016

Yes, ARF6 can regulate actin cytoskeleton dynamics. It interacts with regulators and effectors involved in actin remodeling, such as WAVE complex and WASP, to regulate processes like cell migration, membrane ruffling, and endocytic trafficking.

Does ARF6 play a role in cell adhesion and cell-cell junctions? 05/18/2016

Yes, ARF6 is involved in cell adhesion and the regulation of cell-cell junctions. It contributes to the formation and maintenance of adherens junctions and tight junctions by controlling the trafficking and recycling of junctional proteins, such as E-cadherin.

How is ARF6 involved in endocytosis? 04/18/2016

ARF6 plays a crucial role in clathrin-independent endocytosis, also known as macropinocytosis. It promotes the internalization of extracellular fluids, membrane proteins, and pathogens through the formation of large vesicles called macropinosomes.

What are some diseases or conditions associated with dysregulation of ARF6? 02/25/2016

Dysregulation of ARF6 has been implicated in various diseases and conditions. For example, aberrant ARF6 signaling has been observed in cancer, where it promotes tumor cell invasion and metastasis. ARF6 dysregulation has also been linked to neurodegenerative diseases, such as Alzheimer's and Parkinson's, as well as cardiovascular diseases, including atherosclerosis and hypertension. Additionally, abnormalities in ARF6 activity have been associated with immune disorders and infectious diseases.

How is ARF6 different from other ARF family members? 01/02/2016

ARF6 differs from other ARF family members in its subcellular localization and its role in regulating plasma membrane dynamics. Unlike other ARF proteins, ARF6 is primarily localized to the plasma membrane and plays a crucial role in endocytic and exocytic pathways at the cell surface.

Customer Reviews (8)

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Reviews
03/25/2023

    Their team of experts is readily available to assist you with any inquiries or challenges you may encounter during your experiments.

    07/12/2022

      The ARF6 protein is an exceptional choice that meets the highest standards of quality, making it perfectly suited for your experimental needs.

      05/16/2022

        the ARF6 protein is of the highest quality and perfectly suited for your experimental requirements.

        03/12/2022

          This protein has consistently demonstrated outstanding results, making it an indispensable tool in my research endeavors.

          12/02/2021

            ts stability and structural integrity make it an ideal candidate for studying protein structures at high resolution.

            11/10/2021

              In addition to its exceptional quality, the ARF6 protein is backed by excellent technical support offered by the manufacturer.

              01/21/2020

                In WB experiments, the ARF6 Protein consistently delivers exceptional performance.

                06/24/2017

                  Its exceptional purity and integrity ensure clear and reliable bands, enabling accurate detection and quantification of target proteins.

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