Recombinant human ARG2 cDNA (23-354 aa, Isoform-1, derived from BC029050) fused with 31 aa (T7/His/TEV cleavage site) at N-terminal was expressed in E. coli.
1.0 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, Sucrose and DTT.
MASMTGGQQMGRGHHHHHHENLYFQGGEFVHSVAVIGAPFSQGQKRKGVEHGPAAIREAGLMKRLSSLGCHLKDF GDLSFTPVPKDDLYNNLIVNPRSVGLANQELAEVVSRAVSDGYSCVTLGGDHSLAIGTISGHARHCPDLCVVWVD AHADINTPLTTSSGNLHGQPVSFLLRELQDKVPQLPGFSWIKPCISSASIVYIGLRDVDPPEHFILKNYDIQYFS MRDIDRLGIQKVMERTFDLLIGKRQRPIHLSFDIDAFDPTLAPATGTPVVGGLTYREGMYIAEEIHNTGLLSALD LVEVNPQLATSEEEAKTTANLAVDVIASSFGQTREGGHIVYDQLPTPSSPDESENQARVRI
1. May be used for in vitro ARG2 protein mediated arginine depletion in tumor microenviroment regulation study in various cancer cells with "ProFectin" reagent based intracellular delivery of this protein ( Note: Mitochondrial transit peptide was removed, so this recombinant ARG2 protein will be only located at cytoplasmic one after intracellular protein delivery ).2. May be used as specific protein substrate for kinase and ubiquitin (Sumo pathway) related enzyme functional screening assays.3. May be used for protein-protein interaction mapping.4. As immunogen for specific antibody production.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.