Recombinant Human ARHGDIB, fused with N-His, was expressed in E. coli.
Rho GDP-dissociation inhibitor 2 Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Members of the Rho (or ARH) protein family (see MIM 165390) and other Ras-related small GTP-binding proteins (see MIM 179520) are involved in diverse cellular events, including cell signaling, proliferation, cytoskeletal organization, and secretion. The GTP-binding proteins are active only in the GTP-bound state. At least 3 classes of proteins tightly regulate cycling between the GTP-bound and GDP-bound states: GTPase-activating proteins (GAPs), guanine nucleotide-releasing factors (GRFs), and GDP-dissociation inhibitors (GDIs). The GDIs, including ARHGDIB, decrease the rate of GDP dissociation from Ras-like GTPases.
20mM Tris.Cl, 50mM NaCl, 50% Glycerol, pH8.0.
24.9kDa(including fusion tag)
216aa(including fusion tag)
WB, ELISA, IP, antibody production, protein array
Store at -80°C and avoid repeated freeze-thaw cycles.