Recombinant DNA fragment which encoded for two peptide AG-10 (NRWHSIYITRFG) and AG-32 (TWYKIAFQRNRK) was fully synthesized and fusion to human vitronectin (63-398aa) C-terminal with flexible linker domain (GGGGSGGGGS) for generating VNT-AG10+32 fusion pro
Human CD49f (Integrin a6) has been demonstrated to be specific membrane protein marker for human hematopoietic stem cell (HSC). Two peptide AG-10 (NRWHSIYITRFG) and AG-32 (TWYKIAFQRNRK), which derived from human laminin-111, has been reported to engage with the α6β1 integrin very specifically. To generate a synthetic matrix protein for coating to maintain HSC culture in vitro, AG-10 and AG-32 peptide was fused to human Vitronectin (VNT) C-terminal as recombinant protein. This recombinant VNT-AG10+32 fusion protein may be served as synthetic matrix protein for interaction with α6β1 integrin positive cells.
0.5 mg/ml, sterile-filtered, in 20 mM pH 8.0 Tris-HCl Buffer, with proprietary formulation of NaCl, KCl, EDTA, arginine, DTT and Glycerol.
MASMTGGQQMGRGHHHHHHGNLYFQGGEFGTTNTVAAYNLTWKSTNFKTILEWEPKPVNQVYTVQISTKSGDWKS KCFYTTDTECDLTDEIVKDVKQTYLARVFSYPAGNVESTGSAGEPLYENSPEFTPYLETNLGQPTIQSFEQVGTK VNVTVEDERTLVRRNNTFLSLRDVFGKDLIYTLYYWKSSSSGKKTAKTNTNEFLIDVDKGENYCFSVQAVIPSRT VNRKSTDSPVECMGQEKGEFRE
May be used for in vitro integrain alpha 6 receptor mediated cell differentiation, such as HSC or ES cells regulations, study by using VNT-AG10+32 fusion protein as coating matrix in cell culture.
Keep at -80centigrade for long term storage. Product is stable at 4 centigrade for at least 30 days.