Recombinant Human ATP1B2(68-290aa) fused with His tag at C-terminal was expressed in Insect cells.
ATP1B2, as known as sodium/potassium-transporting ATPase subunit beta-2 isoform 1, is the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ ATPases. It is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The precise function of the beta-2 subunit is not known. This protein is composed of 3 subunits: alpha (catalytic), beta and gamma.
Liquid. In Phosphate Buffered Saline (pH 7.4) containing 10% glycerol.
28-40kDa (SDS-PAGE under reducing conditions.)
ADPDHTPKYQ DRLATPGLMI RPKTENLDVI VNVSDTESWD QHVQKLNKFL EPYNDSIQAQ KNDVCRPGRY YEQPDNGVLN YPKRACQFNR TQLGNCSGIG DSTHYGYSTG QPCVFIKMNR VINFYAGANQ SMNVTCAGKR DEDAENLGNF VMFPANGNID LMYFPYYGKK FHVNYTQPLV
AVKFLNVTPN VEVNVECRIN AANIATDDER DKFAGRVAFK LRINKTHHHH HH
< 1.0 EU per 1 microgram of protein (determined by LAL method)
> 90% by SDS - PAGE
Can be stored at +4 centigrade short term (1-2 weeks). For long term storage, aliquot and store at -20 centigrade or -70 centigrade. Avoid repeated freezing and thawing cycles.
0.5mg/ml (determined by Absorbance at 280nm)