Recombinant Human BCMO1 fused with MYC/DDK tag at C-terminal was expressed in HEK293.
Vitamin A metabolism is important for vital processes such as vision, embryonic development, cell differentiation, and membrane and skin protection. The protein encoded by this gene is a key enzyme in beta-carotene metabolism to vitamin A. It catalyzes the oxidative cleavage of beta,beta-carotene into two retinal molecules.
25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.
> 80% as determined by SDS-PAGE and Coomassie blue staining
Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
>50 ug/mL as determined by microplate BCA method