Recombinant Human BCR/RET(1-426 (BCR); 713-end (RET)) fused with GST tag at N-terminal was expressed in Insect cells.
BCR-RET is a chimaeric fusion of BCR and RET genes generated by a balanced translocation of genes from t(10;22)(q11;q11) and this leads to aberrant activation of RET activity which can transform hematopoietic cells and skew the hematopoietic differentiation program towards the monocytic/macrophage lineage (1). The BCR-RET fusion is frequently found in chronic myelomonocytic leukemia (CMML) cases. The RET fusion genes seem to constitutively mimic the same signaling pathway as RAS mutations frequently involved in CMML.
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
1 year at -70 centigrade from the date of shipment
Store product at –70 centigrade. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.